BlastStation-Local Help

Welcome

Introduction to BlastStation-Local

 FASTA data input

 Job submission

 How to read results

Instructions in detail

 Download database

 Create database

 Remove database

 Change database folder

 Delete jobs

 Backup jobs

 Restore jobs

 Search parameters settings

 Navigation in result window

 Export search results

 Export FASTA data

 Preference settings

 Data folders

Menu Reference

 BlastStation-Local menu

 File menu

 Edit menu

 Job menu

 Tools menu

 Help menu

 

www.BlastStation.com

 

Create database

You can create database files from your own multi-FASTA data or FASTQ data.  Plain, .gz, .Z, and .zip text files are accepted.  FASTA data format is defined here and multi-FASTA data format is defined here.

On the Tools menu, click Create DB.  The Create DB window opens.

Clicking "Select Input File" button will open File Open dialog. Select input  file and click "Open" button.

Or drag and drop input file.

 

Several optional parameters can be specified.  Usually these options do not have to be used.

DB Title: Adds custom title to the database.

Indexing:If input FASTA file has NCBI styled deflines, turn it ON.  Default value is OFF.  It is identical to "-o T" formatdb option.

Advanced Options: formatdb options can be directly specified here.  Please use this option only if you know what you are doing.

Clicking "Create DB" button creates database.  When database is created,  "Database is created" message will be shown.

BlastStation-Local64 Only

When selected input file is large, multiple database files such as nt.00, nt.01, and so on will be created.  At the same time, the alias file, such as nt.nal will be created.  When this alias file is selected as database, Blast+ search over large virtual database can be done.