BlastStation-Local Help

Welcome

Introduction to BlastStation-Local

 FASTA data input

 Job submission

 How to read results

Instructions in detail

 Download database

 Create database

 Remove database

 Change database folder

 Delete jobs

 Backup jobs

 Restore jobs

 Search parameters settings

 Navigation in result window

 Export search results

 Export FASTA data

 Preference settings

 Data folders

Menu Reference

 BlastStation-Local menu

 File menu

 Edit menu

 Job menu

 Tools menu

 Help menu

 

www.BlastStation.com

 

FASTA data input

Double click BlastStation-Local icon, which is in the Applications folder.

Click here. Example FASTA data will be shown in the browser.  Copy and paste it in the Input Data.  If you have a FASTA file on your Mac, you can also drag and drop it to the Input Data area.  FASTA data format is defined here and multi-FASTA data format is defined here.

blastn, blastx, and tblastx are available in the Program section.  This is because the FASTA data are DNA sequences.  If the data are protein sequences, blastp and tblastn will be available.  If blastn is selected, DNA databases will be shown in the Database pull down menu.  If blastp is selected, protein databases will be shown on the other hand.  Two databases, NucleotideExample and ProteinExample, are installed with BlastStation-Local.  You can use them for testing.

BlastStation-Local64 Only

BlastStation-Local64 can select alias files such as .nal or .pal which ties up multiple database file.  Alias files will be made through Create DB or included downloaded database file.

Megablast is the default for blastn program.  Traditional blastn can be selected to uncheck megablast checkbox.