BlastStation2 Help

Welcome

Introduction to BlastStation

 FASTA data input

 Job submission

 How to read results

Remote BLAST Search

 EBI NCBI-Blast

 EBI WU-Blast2

Local BLAST Search

 Download database

 Create database

 BLAST machine selection

Instructions in detail

 Delete jobs

 Backup jobs

 Restore jobs

 Remove database

 Search parameters settings

 Navigation in result window

 Export search results

 Export FASTA data

 Preference settings

 Data directory

Menu Reference

 File menu

 Edit menu

 Job menu

 Tools menu

 Help menu

 

www.BlastStation.com

 

 

How to read results

When BLAST search is finished, the following dialog will be displayed.

Let's click "Yes" to see search results.

Bar graph is shown on the upper half.  The color of the bar is classified by the identity.  Red is 100%, purple is 90% or more, and so on.  The length of the query sequence (606 in this case) is given above the black bar at the top.  You can easily understand which portion of the subject sequence matches with the query sequence.  Move your mouse cursor on the bars.  The color of the bar changes and four numbers will be displayed  as shown below.  The sequence from 19 to 572 of query sequence match with that from 2 to 552 of AY661748.

Table is shown on the lower half, which consists of ID, Accession number, Definition, length, bit score, E Value, and Identity.

In order to display the other search results, click arrows below.

Previous result Next result
Fast backward Fast forward
First result Last result

When you click "Jump", the window below will be shown.  Description of each FASTA file will be listed in this window.  Clicking each description will display corresponding search result.  If you have many descriptions, type in search strings in the text field and click search button to narrow this list.

Display List menu will display Blast results file in the default browser.