How To Build NCBI Blast+ on OS X
This page describes how to build NCBI BLAST+ on OS X 10.8.5 Mountain Lion and OS X 10.9 Mavericks
The procedure  below was confirmed to work fine for NCBI Blast 2.2.28+ on OS X 10.8.5 Mountain Lion and OS X 10.9 Mavericks with Xcode 5.0.1.
 
Up-to-date as of 11/6/2013
 
Click here for Free Trial of BlastStation-Local64 with 64-bit Blast+
 
1. Preparation
1.1. Install Xcode 5.0.1 on your Mac.  You can get Xcode from App Store for free. When Xcode is installed, launch it and install Command Line Tools.
1.2. Download NCBI Blast+ source from ftp://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/2.2.28/ncbi-blast-2.2.28+-src.tar.gz
1.3. Double click ncbi-blast-2.2.28+-src.tar.gz to untar.
1.4. You will get c++ folder in ncbi-blast-2.2.28+-src folder.
2. Configuration and Build process
2.1. Open Applications/Utilities/Terminal.
2.2. Enter the commands below and type return.  make takes a long long time.
2.2.1. cd ncbi-blast-2.2.28+-src/c++
2.2.2. ./configure --without-debug --without-pcre --with-mt --with-flat-makefile --with-ncbi-public --with-build-root=Release --without-ccache --without-caution --without-makefile-auto-update --with-projects=scripts/projects/blast/project.lst --with-internal
      All available options are found in ncbi-blast-2.2.28+-src/c++/src/build-system/configure
2.2.3. make
2.3 All binaries such as blastn and blastp are found in c++/Release/bin.
 
Click here for Free Trial of BlastStation-Local64 with 64-bit Blast+