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How To Build NCBI Blast+ on Mac OS X |
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We investigated why NCBI Blast 2.2.24+ downloaded
from the NCBI ftp site do not work on Mac OS X 10.5 Leopard and
Mac OS X 10.4 Tiger. This page describes the results and
also describes how to build NCBI Blast+ on Mac. |
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The procedure below was confirmed to work
fine for NCBI Blast 2.2.24+ on Mac OS X 10.5 Leopard with Xcode
3.0. |
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We found Apple charges $4.99 for Xcode 4.
Since Xcode 4 is not needed to build NCBI Blast+, we recommend
not to purchase Xcode 4. Please find Xcode 3 in your Mac
OS X DVD which came with your Macintosh or download it from
Apple with free Developer membership. Sign-up page is
http://developer.apple.com/programs/register/ . Xcode
3.2.6 is still available for free download. |
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If you have any questions, please post it to our
facebook page. |
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Up-to-date as of 5/18/2011 |
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| 1. Preparation |
1.1. Install Xcode
3.0 or Xcode 3.2 on your Mac. Xcode
is found in the Mac OS X Installation
DVD or you can download it from Apple
with free Apple Developer membership.
Free membership sign-up page is
http://developer.apple.com/programs/register/
1.2. Download NCBI Blast+ source from
ftp://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/2.2.24/ncbi-blast-2.2.24+-src.tar.gz
1.3. Double click ncbi-blast-2.2.24+-src.tar.gz
to untar.
1.4. You will get c++ folder in
ncbi-blast-2.2.24+-src folder. |
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| 2. Configuration
and Build process |
| 2.1. Open
Applications/Utilities/Terminal. |
| 2.2. Enter the commands below
and type return. make takes a
long long time. |
2.2.1. cd
ncbi-blast-2.2.24+-src/c++
2.2.2.
./configure --without-debug --without-pcre
--with-mt --with-flat-makefile
--with-universal --with-ncbi-public
--with-build-root=Release --without-ccache
--without-caution --without-makefile-auto-update
--with-projects=scripts/projects/blast/project.lst
--with-internal
All available options are found in ncbi-blast-2.2.24+-src/c++/src/build-system/configure
2.2.3. make |
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2.3 All binaries
such as blastn and blastp are found in
c++/Release/bin. |
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3. Why NCBI
Blast+ 2.2.24 downloaded from NCBI does not work on Mac OS
X 10.5 Leopard and 10.4 Tiger. |
3.1. There is a file
named ncbi_package_info in
ncbi-blast-2.2.XX+-universal-macosx.tar.gz
3.2. In this file, compiler version and
configuration parameters used to build
NCBI Blast+ are found.
3.3. NCBI Blast 2.2.23+ was built with gcc 4.0.1 which is bundled with Xcode
3.0 on Mac OS X 10.5. Since -mmacosx-version-min
is not specified in Makefile, the target
Mac OS X is 10.5 or later.
3.4. NCBI Blast
2.2.24+ was built with gcc 4.2.1 which is
bundled with Xcode 3.2 on Mac OS X 10.6.
Since -mmacosx-version-min is not
specified in Makefile, the target Mac OS
X is 10.6 or later. That's why NCBI Blast 2.2.24+ downloaded from NCBI
won't work on Leopard and Tiger.
3.5. NCBI
Blast 2.2.24+ built with Xcode 3.0 on Mac
OS X 10.5 should work on Mac OS X
10.5 and 10.6. And may be on Mac OS X
10.4.
3.6. NCBI Blast 2.2.24+ built on Mac OS X
10.5
is available here
. Download, untar, and copy all binaries in bin folder to the
destination folder. |
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